TMT Proteomics Analysis Services
Advanced Quantitative Proteomics with TMT Technology
Unlock the full potential of your proteomics data with our high-precision TMT analysis service.
Why Choose Our TMT Proteomics Analysis Services?
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High Sensitivity & Accuracy: Detect and quantify even low-abundance proteins with confidence.
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Complex Sample Handling: Analyze intricate biological samples, from cell lines to tissues, with ease.
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Fast Turnaround Times: Receive your results quickly without compromising quality.
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Expert Support: Our team of proteomics specialists is here to guide you through every step.
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Cutting-Edge Technology: We use the latest mass spectrometry equipment for optimal performance.
What is TMT Proteomics Analysis?
Tandem Mass Tag (TMT) technology is a powerful method for quantitative proteomics, allowing researchers to identify and measure protein expression across multiple samples simultaneously. By labeling peptides with TMT reagents, our service enables:
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Multiplexing: Analyze up to 16 samples in a single run, saving time and resources.
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Precise Quantification: Accurately compare protein levels across conditions or treatments.
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Comprehensive Data: Gain insights into protein dynamics, interactions, and post-translational modifications
Whether you're studying disease mechanisms, drug responses, or biomarker discovery, our TMT proteomics service provides the data you need to drive your research forward.
How Our Services Works
Tandem Mass Tag (TMT) technology is a powerful method for quantitative proteomics, allowing researchers to identify and measure protein expression across multiple samples simultaneously. By labeling peptides with TMT reagents, our service enables:
-
Multiplexing: Analyze up to 16 samples in a single run, saving time and resources.
-
Precise Quantification: Accurately compare protein levels across conditions or treatments.
-
Comprehensive Data: Gain insights into protein dynamics, interactions, and post-translational modifications
Whether you're studying disease mechanisms, drug responses, or biomarker discovery, our TMT proteomics service provides the data you need to drive your research forward.
Figure 1 demonstrates the optimization of TMT-to-protein mass ratio for efficient protein labeling. The figure shows: (A) Experimental design with different TMT-to-protein ratios (1:1, 2:1, 4:1, and 8:1), (B) MS spectra of two identified proteins (Phosphocarrier protein HPr and Glutaredoxin 3) showing increased complete labeling at higher ratios, (C) Identification heatmap of labeled species across different ratios, and (D) Violin plots showing the weighted average labeling status moving closer to complete labeling (value 0) as the TMT-to-protein ratio increases. This optimization is crucial for achieving accurate quantification in multiomics proteomics analysis.
Source: Adapted from Figure 1 in Guo, Y., Yu, D., Cupp-Sutton, K. A., Liu, X., & Wu, S. (2022). Analytica Chimica Acta, 1221, 340037.
Frequently Asked Questions
Q: What is the difference between TMT proteomics and other quantitative proteomics methods?
A: TMT (Tandem Mass Tag) proteomics uses isobaric tags to label peptides, enabling the simultaneous identification and quantification of proteins across multiple samples in a single experiment. This targeted, multiplexed approach differs from:
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Label-free quantification, which measures proteins based on spectral counting or peak intensity without tags, offering simplicity but potentially lower precision in complex samples.
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SILAC (Stable Isotope Labeling by Amino acids in Cell culture), which uses metabolic labeling with stable isotopes, typically suited for cell culture studies but less versatile for diverse sample types.
TMT proteomics excels in studies requiring high accuracy and the ability to compare multiple conditions efficiently.
Q: Can you customize services for unique research needs?
A: Yes, we offer fully customizable workflows tailored to your research goals. This includes options such as:
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Protein enrichment for low-abundance targets.
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Post-translational modification (PTM) analysis (e.g., phosphorylation, ubiquitination).
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Custom data analysis pipelines to focus on specific proteins or pathways.
Our team collaborates with you to design a service that meets your project’s unique requirements.
Q: What analytical platforms do you use?
A: We utilize cutting-edge LC-MS/MS platforms, including high-resolution mass spectrometers, optimized for TMT-labeled samples. These systems ensure comprehensive protein identification and accurate quantification, even in complex biological samples.
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